Use of 13C nuclear magnetic resonance to detect binding to target molecules

摘要

Methods of detecting binding of a putative ligand to a ¹³C-enriched target molecule, methods of screening for compounds which bind to a ¹³C-enriched target molecule, methods for calculating the dissociation constant of a ligand compound which binds to a ¹³C-enriched target molecule, and methods employed in the determination of the specific amino acids in a ¹³C-enriched target molecule affected by the binding of a ligand, as well as compounds identified by these screening methods, are provided herewith.