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Method for detecting Helicobacter pylori and heilmanii in fecal and salivary specimen and biopsy material
Method for detecting Helicobacter pylori and heilmanii in fecal and salivary specimen and biopsy material
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机译:粪,唾液标本和活检材料中幽门螺杆菌和Helimanii的检测方法
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摘要
The invention relates to a method for detecting pathogenic organisms, in particular Helicobacter pylori and H.heilmanii, in fecal, salivary and secretory samples by means of a double-antibody sandwich assay. The inventive method is characterized by dissolving or dispersing the sample having the pathogen antigen in a buffer solution and contacting the buffer solution with a solid phase to which at least two primary antibodies are bound, one of which specifically binds to the pathogenic antigen and the other to human immunoglobulin A; washing the solid phase of non-specifically bound proteins and contacting the solid phase with a secondary antibody which specifically binds to pathogenic antigen, and determining the quantity of specifically bound secondary antibodies.
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