The discriminance based on mapping of one point of adjustment controlled level of the transcription and product of gene

摘要

The present invention relates to a kind of to identify the method for mediating one or more areas of one or more interested gene expressions in the genome of interested biology. This method includes identifying the first and second of interested biology, the first interested biology is characterized in that environmental stimulus can be shown measurable reaction, or shows phenotype relevant to the differential gene expression for being relevant to interested process. Second of interested biology is characterized in that lacking the stimulation or without strong reaction as the first described interested biology, or from the first described interested biofacies than with different phenotypes, wherein this different phenotype is related to the interested process, or the biological performance goes out a kind of interested phenotype, the phenotype separates when compared with the first described interested biology, or combinations thereof. Make the first and second of interested biology hybridization, to produce the progeny population of a separation, and extracts RNA from the offspring that each is separated. The gene expression dose of one or more interested genes is quantified, interested one or more genes are related to the reaction to environmental stimulus or interested process. Using the linkage map of one or more label preparative separation offsprings, the relationship on the linkage map between one or more labels and the gene expression of one or more interested genes is determined, and identify one or more quantitative trait locus (QTL). This method further relates to one or more QTLs of identification and one or more interested gene-correlations in the separation offspring for the environmental stimulus for receiving to need. In addition, this method can be used for identifying one or more QTL of the factor corresponding to transcription factor or the one or more interested gene expressions of any control, and separates and characterize the one or more genes being located on one or more QTL.