method of obtaining biologically active compounds of antioxidants and lipid

摘要

the invention belongs to the field of biotechnology and relates to a biologically active compounds: u0443u0431u0438u0445u0438u043du043eu043du043eu0432 (q - 9.10), u03b2 - carotene, u043bu0438u043au043eu043fu0438u043du0430, u044du0440u0433u043eu0441u0442u0435u0440u0438u043du0430, u0444u043eu0441u0444u043eu043bu0438 pids, fatty acids.biomass mushroom Blakeslea trispora with humidity 7 75% u044du043au0441u0442u0440u0430u0433u0438u0440u0443u044eu0442 polar solvent or a mixture of polar and u043du0435u043fu043eu043bu044fu0440u043du043eu0433u043e solvents.the extract is separated from the u0431u0438u043eu0448u0440u043eu0442u0430 by hot filtration. then extract out the crystallization mixture contains polar and u043du0435u043fu043eu043bu044fu0440u043du043eu0433u043e solvents.after separating the crystals from the slurry contains lipid secrete phospholipid. the deposition u0444u043eu0441u0444u043eu043bu0438u043fu0438u0434u043eu0432 by acetone or acetone solvent mixture and u043du0435u043fu043eu043bu044fu0440u043du043eu0433u043e.the solution of neutral lipids u043eu043cu044bu043bu044fu044eu0442 in environment of acetone in water solution of potassium hydroxide. u043eu043cu044bu043bu0435u043du043du0443u044e faction u0433u0438u0434u0440u043eu043bu0438u0437u0443u044eu0442 sulphuric acid to obtain a mixture of fatty acids.u043du0435u043eu043cu044bu043bu0435u043du043du0443u044e faction u043fu0435u0440u0435u0440u0430u0441u0442u0432u043eu0440u044fu044eu0442 in u0433u0435u043au0441u0430u043du0435 and u043au0440u0438u0441u0442u0430u043bu043bu0438u0437u0443u044eu0442 u044du0440u0433u043eu0441u0442u0435u0440u0438u043d. after separation on the column with u044du0440u0433u043eu0441u0442u0435u0440u0438u043du0430 leachate are u0430u0434u0441u043eu0440u0431u0435u043du0442u043eu043c.with the u0430u0434u0441u043eu0440u0431u0435u043du0442u0430 u044du043bu044eu0438u0440u0443u044eu0442 non-polar solvent side fractions, and then with a mixture of a polar solvent and u043du0435u043fu043eu043bu044fu0440u043du043eu0433u043e u044du043bu044eu0438u0440u0443u044eu0442 u0443u0431u0438u0445u0438u043du043eu043du043eu0432 fraction.the solution u0443u0431u0438u0445u0438u043du043eu043du043eu0432 u0443u043fu0430u0440u0438u0432u0430u044eu0442 and u043au0440u0438u0441u0442u0430u043bu043bu0438u0437u0443u044eu0442 them in u044du0442u0438u043bu043eu0432u043eu043c ethanol. the process of crystallization residue summary (q - 9.10) u0443u0431u0438u0445u0438u043du043eu043du043eu0432 separate u0444u0438u043bu044cu0442u0440u043eu0432u0430u043du0438u0435u043c.