E. coli enterotoxin II signal peptide and a microorganism expressing a fusion protein of the MST peptide and a heterologous protein, such as human growth hormone

摘要

An E.coli enterotoxin II signal peptide shown below characterized in that at least one of the 2nd, 4th, 5th, 12th, 20th and 22nd amino acids is replaced by another amino acid, with at least either 2nd or 4th amino acids being lysine. Also disclosed is a process for producing a heterologous protein in a microorganism comprising culturing a microorganism transformed with an expression vector (comprising a gene that encodes the E.coli enterotoxin II signal peptide) to produce and secrete the heterologous protein to periplasm and subsequently recovering the protein from the periplasm.