POLYPEPTIDE WITH DIKINASE ORTHOPHOSPHATE PYRUVATE ACTIVITY, DNA ENCODING THE SAME, RECOMBINANT VECTOR WITH SAID DNA AND PROCESS FOR MODIFYING A PLANT BY MEANS OF SAID VECTOR

摘要

The present invention relates to a polypeptide with dikinase orthophosphate pyruvate activity, stable at low temperatures, containing the amino acid sequence from the 832 amino acid residue to the 955 amino acid residue, of the amino acid sequence number 5, excepting the fact that one or more amino acid residues are added, deleted or substituted, this conferring stability at low temperatures. The invention also relates to a cloned DNA encoding the previously defined polypeptide which has the nucleotide sequence presented in the serv. number 5, excepting the fact that one or more nucleotides are added, deleted or substituted. The said DNA is introduced in a recombinant vector which has a structure presented in figure 1 or 3, and which may express, in the host cell, a polypeptide having dikinase orthophosphate pyruvat activity, stable at low temperatures. The invention presents a process for modifying a plant of Flaveria bidentis or corn, for providing the stability at low temperatures, process where a previously defined recombinant vector is used as modifying agent.