POLYPEPTIDE HAVING COLD-STABLE PYRUVATE, ORTHOPHOSPHATE DIKINASE ACTIVITY, DNA ENCODING THE SAME AND RECOMBINANT VECTOR AND TRANSFORMED PLANTS CONTAINING THE DNA

摘要

A novel polypeptide having a cold-resistant pyruvate phosphate dikinaseactivity; a cloned DNA coding for the polypeptide; and a recombinant vectorcontaining the DNA, each as a means for imparting cold resistance to plants.The polypeptide is either the following polypeptide (1) or polypeptide (2) andhas such an amino acid sequence that at least one amino acid residue presentin the region having a length of one-sixth of the whole length starting withthe C-terminus has been replaced by another amino acid residue and also has acold-resistant pyruvate phosphate dikinase activity: 1) a polypeptide havingan amino acid sequence represented by any of the SEQ ID no:1 to no:4 in thesequence listing and also having a pyruvate phosphate dikinase activity; and2) a polypeptide having such an amino acid sequence that at least 50 % thereofis homologous with the amino acid sequence mentioned in item 1) and alsohaving a pyruvate phosphate dikinase activity.