VIRULENCE GENES IN THE VGC2 REGION OF SALMONELLA.

摘要

A MICROORGANISM WITH ADAPTATION REDUCED TO A PARTICULAR ENVIRONMENT, IDENTIFIED WITH A METHOD THAT INCLUDES THE STEPS OF: (1) OBTAINING MULTIPLE MICROORGANISMS EACH OF WHICH IS INDEPENDENTLY MUTED BY INACTIVATION BY INSERTION OF A GENE UNIQUE MARKER OF MODE THAT EACH MUTANT CONTAINS A SEQUENCE OF DIFFERENT MARKER, OR CLONES OF SUCH MICROORGANISM; (2) OBTAIN INDIVIDUALLY A STORED SAMPLE OF EACH MUTANT PRODUCED IN STEP (1) AND OBTAIN INDIVIDUALLY STORED NUCLEIC ACIDS CONTAINING THE UNIQUE MARKER SEQUENCE OF EACH INDIVIDUAL MUTANT; (3) ENTER MULTIPLE MUTANTS PRODUCED THROUGH STEP (1) IN SUCH PARTICULAR ENVIRONMENT, AND ALLOW THE MICROORGANISMS ABLE TO GROW IN THAT ENVIRONMENT TO DO IT; (4) REMOVE MICROORGANISMS FROM SUCH ENVIRONMENT OR A SELECTED PART OF THE SAME, AND ISOLATE THE NUCLEIC ACID FROM THE EXTRACTED MICROORGANISMS; (5) COMPARE THE MARKER SEQUENCES OF THE NUCLEIC ACIDS ISOLATED IN STEP (4) WITH THE UNIQUE MARKER SEQUENCE OF EACH INDIVIDUAL MUTANT STORED IN STEP (2); AND (6) SELECT AN INDIVIDUAL MUTANT THAT DOES NOT CONTAIN ANY OF THE MARKER SEQUENCES ISOLATED IN STEP (4). IN ADDITION, THE INVENTION REFERS TO: AN ISOLATED MICROORGANISM GENE, A VACCINE THAT INCLUDES MICROORGANISM, A POLIPEPTIDE CODIFIED BY THE GENE, A METHOD FOR IDENTIFYING COMPOUNDS THAT INTERFER WITH THE FUNCTION OF THE GENE, eg: BY ANTISENTIDE. THE MICROORGANISM IS EXAMPLED WITH SALMONELLA TYPHIMURIUM AND ITS GEN VGC2.