Method for determining chitinase activity based on usage of fluorescent substrates involves incubation of sample of enzyme with chitinase activity or of preparation containing such enzyme in presence of appropriate substrate

摘要

The method for determining chitinase activity based on the usage of fluorescent substrates involves the incubation of a sample of an enzyme with chitinase activity or of a preparation containing such an enzyme in the presence of an appropriate substrate. The substrate is marked with a fluorochrome, under conditions in which are formed the products of the reaction catalyzed by the chitinase activity. The emission of fluorescence is then measured.