ANTIBODIES TO LIGAND ANALOGUES AND THEIR UTILITY IN LIGAND-RECEPTOR ASSAYS

摘要

Means for the detection of free ligand analogue conjugate in fluids fromcompetitive ligand-receptor assay processes. Li-gand analogue antibodies that bind the ligand analogue conjugate withsubstantially greater affinity than their affinity for targetligand are selected and used in competitive ligand-receptor assay processes tobind the free fraction of the ligand analogue conju-gate. This means permits the detection of the free fraction of ligand analogueconjugate even in the presence of substantially high-er concentrations of free target ligand. For the purposes of the presentinvention, ligand analogue antibodies are antibodies thatexhibit at least 100 X greater affinity for the ligand analogue conjugatecompared to their affinity for the target ligand.