首页> 外国专利> METHODS AND OLIGONUCLEOTIDES FOR THE DETECTION OF SALMONELLA SP., E. COLI O157:H7, AND LISTERIA MONOCYTOGENES

METHODS AND OLIGONUCLEOTIDES FOR THE DETECTION OF SALMONELLA SP., E. COLI O157:H7, AND LISTERIA MONOCYTOGENES

机译：方法和寡核苷酸检测沙门氏菌，大肠杆菌O157：H7和单核细胞增多性单胞菌

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摘要

A method for detecting a Salmonella species, E. coli O157:H7, or Listeriamonocytogenes is disclosed. The method involves amplifying a genomicnucleotide sequence of a corresponding species and detecting the amplificationproduct. Various primers and probes that can be used in the method are alsodisclosed. In one embodiment, the amplification step of the method isaccomplished by real-time PCR and the amplification product is detected byfluorescence resonance energy tranfer using a pair of labeled polynucleotides.

机译：一种检测沙门氏菌，大肠杆菌O157：H7或李斯特菌的方法公开了单核细胞增生病。该方法涉及扩增基因组相应物种的核苷酸序列并检测扩增产品。还可用于该方法的各种引物和探针披露。在一个实施方案中，该方法的扩增步骤为通过实时PCR完成，扩增产物通过使用一对标记的多核苷酸的荧光共振能量转移。

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公开/公告号CA2451498A1

专利类型
公开/公告日2003-01-03

原文格式PDF
申请/专利权人 MARSHFIELD CLINIC;
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申请/专利号CA20022451498
发明设计人 ELLINGSON JAY L. E.;
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申请日2002-06-21
分类号C12Q1/68;C07H21/04;C12P19/34;
国家 CA
入库时间 2022-08-21 23:58:25

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