METHOD TO DETECT SPLICE VARIANTS OF THE CALCINEURIN GENE

摘要

A method for detecting isoforms and/or splice variants of Calcineurin or itssubunits is established. The inventive method is especially useful fordifferentiation between the various isoforms of Calcineurin. Herein apolymerase chain reaction of nucleic acids of mammalian tissue is performedand the corresponding products are evaluated. Especially prefered is a reversetranscribed polymerase chain reaction. For the polymerase chain reactionprimers for the isoforms and/or splice variants of Calcineurin are used. In aprefered embodiment isoforms and/or splice variants of the catalytic subunitCalcineurin-A are detected.