COLORIMETRIC GENETIC TEST FOR CLINICALLY SIGNIFICANT TNF POLYMORPHISM AND METHODS OF USE THEREOF

摘要

The present invention discloses a method and diagnostic kit used to identify polymorphisms. The method makes use of a minor groove binder and shorter hybridization probes so that polymorphisms, including polymorphisms of only a single nucleotide, may be detected. The present invention additionally discloses methods using hybridization conditions which increase the sensitivity of each individual nucleotide within the region being assayed. The invention additionally includes a diagnostic kit which supplies the necessary materials as well as instructions so that the method disclosed herein may be practiced in a time efficient manner.