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GENERATION OF TRANSGENIC MICE BY TRANSGENE-MEDIATED RESCUE OF SPERMATOGENESIS
GENERATION OF TRANSGENIC MICE BY TRANSGENE-MEDIATED RESCUE OF SPERMATOGENESIS
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机译:转基因介导的精子增生产生转基因小鼠
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摘要
The present invention provides transgenic mice and nucleic acid constructs and methods for creating them. The transgenic mice are generated preferably by electroporation-mediated transfection of a nucleic acid construct into the testes of a sterile spermatogenesis-deficient male mouse. The construct includes DNA encoding a spermatogenesis rescue factor resulting in rescue of spermatogenesis while contemporaneously transferring a nucleic acid of interest for expression in the transgenic mouse so that progeny sired by the transgenic mouse also harbor the transgene. Certain embodiments of the invention also utilize (a) the salmonid derived Sleeping Beauty transposition system to augment genomic integration of the transgene and/or (b) the Cre/loxP recombination system for excision from sired progeny of the DNA responsible for spermatogenesis rescue.
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