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APPLICATION OF BIOINFORMATICS FOR DIRECT STUDY OF UNCULTURABLE MICROORGANISMS
APPLICATION OF BIOINFORMATICS FOR DIRECT STUDY OF UNCULTURABLE MICROORGANISMS
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机译:生物信息学在不可破坏微生物研究中的应用
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摘要
A method for identifying unculturable microorganisms in which at least one bacterial cell from an environmental sample containing a plurality of microorganisms is isolated, at least one DNA fragment from the at least one bacterial cell is amplified, cloned into at least one E. coli vector and sequenced, resulting in identification of at least one DNA sequence. The at least one DNA sequence is compared with existing DNA databases, resulting in identification of the at least one DNA sequence as derived from either an unculturable microorganism or a known microorganism.
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