METHOD FOR SIMULTANEOUS DETECTION OF MULTIPLE FLUOROPHORES FOR IN SITU HYBRIDIZATION AND CHROMOSOME PAINTING

摘要

A spectral imaging method for detecting and analyzing fluorescent in situ hybridizations (fig. 5) employing numerous chromosome paints (fig. 9) and/or loci specific probes each labeled with a different fluorophore or a combination of fluorophores, the method is highly sensitive both in spatial and spectral resolutions (fig. 6) and is capable of simultaneous detection of dozens of fluorophores or combinations of fluorophores (fig. 7). The method of the present invention can be used for the detection of fluorescently painted complete sets of chromosomes and/or multiple loci from a species such as human (fig. 10).