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METHOD FOR CULTURING AND EXPANSION OF MAMMALIAN UNDIFFERENTIATED EPIDERMAL KERATINOCYTES EXHIBITING STEM CELL CHARACTERISTICS
METHOD FOR CULTURING AND EXPANSION OF MAMMALIAN UNDIFFERENTIATED EPIDERMAL KERATINOCYTES EXHIBITING STEM CELL CHARACTERISTICS
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机译:具有干细胞特性的哺乳动物未分化表皮角化细胞的培养和扩增方法
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摘要
The invention disclosed relates to the culturing of mammalian e.g. human cells, and in particular to the culturing and expansion of substantially undifferentiated human epidermal keratinocytes exhibiting stem cell-like characteristics, and in co-culture with human dermal fibroblasts utilizing a low calcium serum free and animal by-product free medium derived from a commercially available medium. The medium consists of a commercially available basal medium, and a single fibroblast growth factor (FGF) or a mimic thereof e.g. FGF7/KGF. A method is also disclosed for treating mammalian e.g. human skin wound injuries, by applying to the wound an effective amount of substantially pure mammalian e.g. human epidermal stem cell-like keratinocytes in a substantially undifferentiated state, and optionally additionally, dermal fibroblasts e.g. human dermal fibroblasts. Both cell types can be grown separately or in a co-culture for application purposes.
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