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GENES FOR ALTERING MITOCHONDRIAL FUNCTION AND FOR HYBRID SEED PRODUCTION

机译:改变线粒体功能和杂交种子生产的基因

摘要

The present invention relates to isolated nucleic acid molecules which restore fertility to cytoplasmic male sterile plants and modify expression of toxic mitochondria proteins by the plant. The present invention also relates to methods of identifying a candidate plant suitable for breeding with a cytoplasmic male sterile plant and methods of identifying a candidate gene restoring fertility in plants by analyzing for the candidate plant and candidate gene, respectively, for the presence of the nucleic acid molecule of the present invention. Also disclosed are methods of producing hybrid plant seed, methods of directing gene expression to plant mitochondria, and method of expressing a gene preferentially in roots of a plant. Promoters and terminators from plant genes which restore fertility to cytoplasmic male sterile plants and modify expression of toxic mitochondria proteins are also disclosed. Finally, methods of producing plants with a cytoplasmic male sterile plant restoration system are disclosed.
机译:本发明涉及分离的核酸分子,其可恢复对细胞质雄性不育植物的繁殖力并改变植物毒性线粒体蛋白的表达。本发明还涉及鉴定适合用细胞质雄性不育植物育种的候选植物的方法,以及通过分别分析候选植物和候选基因的核酸存在来鉴定恢复植物育性的候选基因的方法。本发明的酸分子。还公开了产生杂种植物种子的方法,将基因表达引导至植物线粒体的方法以及优先在植物根部表达基因的方法。还公开了来自植物基因的启动子和终止子,其恢复了对细胞质雄性不育植物的育性并改变了毒性线粒体蛋白的表达。最后,公开了用细胞质雄性不育植物恢复系统生产植物的方法。

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