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ENZYMATIC PRODUCTION OF DIFRUCTOSE DIANHYDRIDE IV FROM SUCROSE AND RELEVANT ENZYMES AND GENES CODING FOR THEM
ENZYMATIC PRODUCTION OF DIFRUCTOSE DIANHYDRIDE IV FROM SUCROSE AND RELEVANT ENZYMES AND GENES CODING FOR THEM
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机译:从蔗糖和相关酶中酶法生产二果糖二酐及其基因编码
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摘要
Disclosed is the production of difructose dianhydride IV from sugar. A sugar solution is subjected to reaction at room temperature or lower in an acidic buffer of pH 3.0-7.0 in the presence of a levansucrase derived from Z. mobilis to produce levan. The levansucrase is prepared by culturing E. coli BL21 (DE3)/pEL12 (KCTC 8661), harvesting and homogenizing the cells, and isolating levansucrase from the cell homogenate. Levan is purified from the reaction solution and subjected to reaction at 25-50° C. for 3-10 hours in an acidic buffer of pH 3.0-7.0 in the presence of a levan fructotransferase to produce difructose dianhydride IV. The levan fructotransferase is obtained from E. coli JUD81 (KCTC 0877BP). Also, disclosed are a gene coding for the levan fructotransferase and an expression vector pUDAF81 carrying the gene.
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