Crystals of the human TRAIL/sDR5 (TNF-related apoptosis-inducing ligand/extracellular domain of DR5 receptor) complex, their three-dimensional structure and uses thereof

摘要

The present invention relates to a crystal of TRAIL (TNF related apoptosis including ligand)-sDR5 complex and TRAIL mutant protein. Particularly, the present invention relates to a method for preparing the crystal of human derived TRAIL protein and TRAIL receptor protein sDR5 complex and the crystal of TRAIL-sDR5 complex prepared by the method. The present invention also relates to a three-dimensional structure of TRAIL protein in the form of complex with TRAIL receptor sDR5 as well as TRAIL protein itself, and specific residues existed in the contact central region of AA" loop, which is involved in a specific polar interaction with sDR5 and plays an important role in the receptor binding affinity and the cytotoxic activity by analyzing the deletion mutant of TRAIL protein.