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CARRIER BEADS FOR CELL CULTURE BEARING PARTICULAR PROPERTIES AND PRODUCTION THEREOF
CARRIER BEADS FOR CELL CULTURE BEARING PARTICULAR PROPERTIES AND PRODUCTION THEREOF
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机译:带有细胞培养特性的载体珠及其生产
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摘要
The invention concerns a method for continuously obtaining two-dimensional microcarrier beads (2D-MS) displaying particular functional characteristics for anchorage-dependent cell culture (CAD), comprising a succession of steps which consist in: continuously producing polymer film rolls from a specific polymer granules; activating said polymer film by any means generating reactive groups; covalent grafting between the activated polymer film and a polymer, copolymer or a macromolecule of interest; if required, washing to eliminate the monomers which have not been consumed and fixed on the film, followed by drying the grafted film; and finally cutting the grafted polymer film by continuous punching, the size of the punch being selected according to that of the dimension desired for the two-dimensional microcarrier beads.
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