NOVEL 'OXY' CMS BRASSICA NAPUS PLANT CORRECTED FOR CHLOROSIS USING HEXAPLOID BRIDGING MATERIAL GENERATED THROUGH PROTOPLAST FUSION

摘要

The present invention relates to a novel cytoplasmic male sterile (CMS) Brassica napus plant containing improved 'oxy' cytoplasm i.e. chloroplast from Brassica oleracea and recombinant mitochondria from Brassica oxyrrhina, a process for developing the said 'oxy' CMS Brassica napus plants by using hexaploid 'oxy' CMS Brassica plants, obtained through somatic hybridization, as the bridging material and back-crossing these for at least five generations to transfer the novel 'oxy' CMS character to Brassica napus plants and a method for producing the improved hexaploid male sterile 'oxy' CMS Brassica plants mediated through protoplast fusion and regeneration of the somatic hybrids and to the transfer of this improved 'oxy' cytoplasm to tetraploid Brassica napus (AACC) plants through conventional back-cross breeding for five generations.