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A PROCESS FOR THE FERMENTATIVE PREPARATION OF D-PANTOTHENIC ACID USING CORYNEFORM BACTERIA WITH DELETED PCK (PHOSPHOENOLPYRUVATE CARBOXYKINASE, 4.1.1.49) GENE
A PROCESS FOR THE FERMENTATIVE PREPARATION OF D-PANTOTHENIC ACID USING CORYNEFORM BACTERIA WITH DELETED PCK (PHOSPHOENOLPYRUVATE CARBOXYKINASE, 4.1.1.49) GENE
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机译:一种用去氧PCK(磷酸戊二酸酯羧激酶,4.1.1.49)基因与细菌一起发酵制备D-泛酸的方法
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摘要
The invention provides a process for preparing D-pantothenic acid by the fermentation of coryneform bacteria in which bacteria are used in which the nucleotide sequence (pck gene) coding for phosphoenolpyruvate carboxykinase (EC 4.1.1.49) is attenuated, wherein the following steps are performed: a) fermentation of D-pantothenic acid producing bacteria in which at least the gene coding for phosphoenolpyruvate carboxykinase is attenuated, b) enrichment of D-pantothenic acid in the medium or in the cells of the bacteria, and c) isolation of the D-pantothenic acid produced.
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