Method for generating a subtracted cDNA library and uses of the generated library

摘要

This invention provides a method of generating a subtracted cDNA library of a cell comprising: a) generating a cDNA library of the cell: b) isolating double-stranded DNAs from the cDNA library: c) releasing the double-stranded cDNA inserts from the double-stranded DNAs: d) denaturing the isolated double-stranded cDNA inserts: e) hybridizing the denatured double-stranded cDNA inserts with a labelled single-stranded nucleic acid molecules which are to be subtracted from the cDNA library: and f) separating the hybridized labeled single-stranded nucleic acid molecule from the double-stranded cDNA inserts, thereby generating a subtracted cDNA library of a cell. This invention also provides different uses of the subtracted library.