EXPRESSION METHOD OF hGM-CSF AND RECOMBINANT hGM-CSF PROTEIN USING BY PLANT CELL SUSPENSION CULTURE

摘要

PURPOSE: Provided are a method for producing human granulocyte-macrophage colony stimulating factor and a recombinant human granulocyte-macrophage colony stimulating factor protein by using the plant cell suspension culture, thereby cheaply mass producing the human granulocyte colony stimulating factor. CONSTITUTION: A recombinant vector pMY064 contains a gene encoding human granulocyte-macrophage colony stimulating factor(hGM-CSF) having the nucleotide sequence of SEQ ID NO: 1. A transformed plant cell is produced by transforming a plant cell with the recombinant vector pMY064, wherein the transformed plant cell is Nicotiana tabacum pMYO64(KCTC 0670BP). The recombinant human granulocyte-macrophage colony stimulating factor protein is produced by the suspension culture of the plant cell Nicotiana tabacum pMYO64(KCTC 0670BP), wherein the suspension culture is carried out by further addition of 0.1 to 5 g/l of polyvinyl pyrrolidine into an MS medium.