Liquid Culture Method of Pine Mushroom Mycellium

摘要

The present invention relates to a liquid culture method of high-quality pine mycelium having a unique flavor of wild pine. In the present invention, the synthetic medium component is added to an extract mixture mixture of hardwood extract and pine extract in a ratio of 3: 7, and then the pH is adjusted to 5 to 8 to inoculate sterile mycelium on autoclaved plate medium aseptically. Preculture to 10 days; Sterilely inoculating the precultured mycelium on a liquid medium of the same component as the preculture medium, followed by cancer culture while stirring and aeration for 2 to 6 days at 20 to 35 ° C .; And aeration culture for 4 to 10 days in the same medium as the cancer culture step.