PRIMER SET SPECIFIC TO GENUS STREPTOMYCES rpoB GENE SEQUNCE AND IDENTIFICATION METHOD OF GENUS STREPTOMYCES BY USING THE SAME

摘要

PURPOSE: A primer set specific to genus Streptomyces, rpoB gene fractions, and an identification method of genus Streptomyces by using the same are provided, thereby identifying the genus Streptomyces easily and cheaply. CONSTITUTION: The primer set specific to genus Streptomyces is selected from the group consisting of primers having the nucleotide sequences of SEQ ID NOs: 1 and 2, specifically amplifying RNA polymerase B(rpoB) subunit gene. The polynucleotide is selected from the nucleotide sequences of SEQ ID NOs: 3 to 164 and comprises 306 bp of the rpoB gene fraction of the genus Streptomyces. The identification method of genus Streptomyces comprises the steps of: (i) amplifying the rpoB gene fraction of a strain using the primer set specific to the genus Streptomyces; (ii) sequencing the amplified ropB gene fractions; and (iii) comparing the nucleotide sequences analyzed in the step (ii) and the rpoB gene sequence.