The therapeutical composion for diabetes from Lentinus edodes exo-polymer produced from submerged mycelial culture and method for isolation thereof

摘要

PURPOSE: A method for separating the Lentinusedodes-derived extracellular polysaccharide by the liquid culture of mycelium and a composition containing the extracellular polysaccharide for treating diabetes are provided, wherein the Lentinusedodes-derived extracellular polysaccharide shows an excellent blood sugar lowering effect. CONSTITUTION: The method comprises the steps of culturing Lentinusedodes in a potato/dextrose medium by using a rotary flask shaker under the condition of 120 rpm and 25 deg.C for 10 days; pulverizing the Lentinusedodes culture medium under the aseptic condition and injecting the pulverized one to the culture medium with a concentration of 1 %(v/v); liquid culturing the medium solution containing the pulverized one by using a rotary flask shaker under the condition of 120 rpm and 25 deg.C for 24 hours; centrifuging the liquid culture solution with 10,447 x g/20 min to obtain supernatant, precipitating the obtained supernatant in ethanol and centrifuging the precipitated one; and lyophilizing the obtained precipitate and obtaining a suspension by using distilled water.