Method of Preparing Mature Dendritic Cell-Vaccine for Immunotherapy

摘要

PURPOSE: A method for preparing mature dendritic cell-vaccine for immunotherapy is provided, thereby exhibiting the disease-specific antigen on the surface of a cell, so that antigen-specific T cell immune response can be strongly induced. CONSTITUTION: A fusion antigen comprises (a) proteins or peptides having antigenicity; and (b) cellular membrane-penetrable peptides coupled to the N-terminal of the proteins or peptides and having the substantially identical amino acid sequence of SEQ ID NO: 1, wherein the proteins or peptides having antigenicity are cancer-related proteins, a portion of proteins or peptides derived from proteins selected from prostate acid phosphatase, HER-2/neu(Herstatin), CEA(Carcinoembryonic antigen), MUC-1, MART-1, MAGE-1, MAGE-3, gp100, tyrosinase, E7 of human pamiloma virus, p53 protein and Ras. A method for preparing mature dendritic cell-vaccine for immunotherapy comprises comprising culturing immature dendritic cell with the cellular membrane-penetrable peptide-fused fusion antigen, wherein the immature dendritic cell is derived from monocytes; the monocytes are obtained from peripheral blood; and the immature dendritic cell is obtained by differentiation of monocytes in a medium containing (i) cytokine selected from interleukin-4(IL-4), granulocyte macrophage colony stimulating factor, IL-13, IL-7, transforming growth factor-beta 1, tumor necrosis factor-alpha and a mixture thereof, or (ii) cell signal material selected from calcium ionophore and phorbol ester.