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KIT FOR DETECTING ANTIBODIES TO ENCEPHALOMYELITIS VIRUS IN POULTRY

机译:用于检测家禽脑脊髓炎病毒抗体的试剂盒

摘要

FIELD: veterinary medicine. SUBSTANCE: the suggested kit contains purified and inactivated antigen of encephalomyelitis virus in poultry (EP) immobilized upon solid carrier, dry positive hen's blood serum to EP virus, dry negative hen's blood serum and dry antispecific immunoperoxidase conjugate against hen's immunoglobulins additionally containing carboxylcontaining cationite CB-4P-2. Every of mentioned components and cationite CB-4P-2 are applied at the following ratio, weight%: 2:98 - 15:85. Out of nonspecific components the suggested kit contains tris- buffer solution, phosphate-citrate buffer, orthophenylene diamine dyestuff or 2,2-azino-di [3-ethyl] benzthiazolinesulfonic acid, solution to stop reaction dying process, detergent twin-20 and washing solution. The kit in question is of high activity, specificity, low cost price and high stability at storage. EFFECT: higher efficiency of detection. 11 cl, 6 tbl
机译:领域:兽医学。物质:建议的试剂盒包含固定在固体载体上的家禽脑脊髓炎病毒的纯化和灭活抗原(EP),干燥的母鸡血清对EP病毒,干燥的母鸡血清和干燥的抗母鸡免疫球蛋白的抗特异性免疫过氧化物酶结合物,另外还包含含羧基的阳离子CB -4P-2。所提及的每种成分和阳离子沸石CB-4P-2均以以下重量比(2:98-15:85)施用。在非特定组分中,建议的试剂盒包含tris-缓冲液,柠檬酸磷酸盐缓冲液,邻苯二胺染料或2,2-叠氮基二[3-乙基]苯并噻唑啉磺酸,用于终止反应染色过程的溶液,洗涤剂twin-20和洗涤解。所讨论的试剂盒具有高活性,特异性,低成本和高储存稳定性。效果:更高的检测效率。 11厘升,6汤匙

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