A method for the detection of hydrogen peroxide, water substance peroxide image ends systems, peroxidases and oxidases or peroxidatively active substances

摘要

Secondary (hetero)arylmethylamine derivatives are used as chromogenic reagents in peroxidase/hydrogen peroxide assay systems. Assay for hydrogen peroxide, hydrogen peroxide-producing systems, peroxidases, oxidases or substances with peroxidase activity comprises using a chromogenic reagent of formula (I) and quantifying the resulting color change or colored precipitate: Z = carbon-bearing substituent with n C-N bonds, preferably an optionally substituted mono-, di- or trivalent aromatic or heteroaromatic group; n = 1 or more; a = 0 or 1; R4, R5 = H; R2, R3 = phenyl with 0-5 substituents; heteroaryl, e.g. pyridyl with 0-4 substituents or pyrrolyl, furyl or thienyl with 0-3 substituents; or metallocenyl, e.g. ferrocenyl with 0-9 substituents; where the substituents are selected from OH, alkoxy, aryloxy, acyloxy, alkyl, aryl, heteroaryl, halogen, NO2, COOH, alkoxycarbonyl, aryloxycarbonyl, NH2, aminoalkyl and aminoaryl; A = an anion. R1 = not defined.