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Production of modified hydroxy-polymer for use in immobilisation of proteins comprises reacting a solution of hydroxy-polymer in polar aprotic solvent with carboxylic acid, azide reagent and organic base
Production of modified hydroxy-polymer for use in immobilisation of proteins comprises reacting a solution of hydroxy-polymer in polar aprotic solvent with carboxylic acid, azide reagent and organic base
Production of modified hydroxy-polymers with carbamate-linked functional groups comprises reacting a solution of hydroxy-polymer in polar aprotic solvent with carboxylic acid(s), an azide group-transferring reagent and an organic base at elevated temperature. Production of modified hydroxy-polymers with functional group(s) attached to the polymer backbone by carbamate groups comprises reacting a solution of the hydroxy-polymer in polar aprotic solvent(s) with: (a) A carboxylic acid or a mixture of different carboxylic acids; (b) An azide group-transferring reagent; and (c) An organic base at elevated temperature and then working up the resulting polymer, reactants (a), (b) and (c) being used in the amounts required to achieve a controlled degree of modification of the polyhydroxy compound.
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