A method for the application of nucleosides and / or nucleotides on functionalized surfaces as well as a method for the determination of coupling yields it is necessary in the synthesis of nucleotides

摘要

The present invention relates to a process for the application of nucleosides and / or nucleotides on functionalized surfaces, which are provided with reactive functional groups, with the reactive functional groups in a first step with suitable derivatized nucleosides and / or nucleotides are brought to reaction and in a second step with a protecting groups reagent be reacted, so that a reaction product of the subsequent reaction with electromagnetic radiation interacts in such a way that it can be determined quantitatively. The present invention further relates to a method for the determination of coupling yields it is necessary in the synthesis of nucleotides, wherein the free 3 '- or 5' - hydroxy group of a selected nucleoside and / or nucleotide with a compound of formula (i) is reacted dollars f1 where l is a typical suitable leaving group, px a phosphoramidite, a h - phosphonate, a phosphonic acid esters or a phosphorous triester, y = o or s, n is a nucleoside - or a nucleotide derivative is selected from the following general formulae (ii), (iii), (iv): dollar f2