Arrangement and use of a device to determine the concentration of haemoglobin derivatives in an unfinished, unfinished full blood sample

摘要

Apparatus to determine the concentration of haemoglobin derivatives in an undiluted and non-haemolised whole blood sample (2) in a dish (1), uses 2 measurement geometries for spectral register of a collimated central beam (7) and 1 scattered beam (9). The detection of the central beam (7), projecting from an initial measurement site (a0) at the dish (1) occurs at an initial measurement point (m0) on the axis (4') of the primary beam (4). The detection of the scattered beam (9) is either on a projection from the first measurement site (a0) at a second measurement point (m1) or from a second measurement site (a1) at the dish (1) at the first measurement point (m0). The axes (4',9') of the central (7) and scattered (9) beams form an included angle ( alpha 1). The evaluation system (10) has a unit (11) to compute the concentration of a number of haemoglobin derivatives in 1 measurement geometries to give 2 of the number of measured values and a given calibration matrix. Also claimed is a method where a primary beam is directed at the sample, with a number of monochrome light components of different wavelengths. The beam passing through the sample is detected at a measurement point on the axis of the primary beam, where there are a number of absorption values A0( lambda ) in the central beam. The beam passing through the sample is detected at least at one further measurement point on the axis of a scattered beam, at an angle ( alpha 1) to the axis of the primary beam, to give a further set of absorption values A alpha i( lambda ) in the scattered beam. The concentration of a number of haemoglobin derivatives is computed as a function of the absorption values and a given calibration matrix.