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promoter / terminator gene of candida boidinii of formatdehydrogenase
promoter / terminator gene of candida boidinii of formatdehydrogenase
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机译:脱氢酶的博伊丁氏假丝酵母启动子/终止子基因
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摘要
A promoter of a formate dehydrogenase gene of C. boidinii which substantially contains a consecutive base sequence of 190 bp or more picked out in the base sequence represented by SEQ ID NO: 1 in the Sequence Listing; a formate dehydrogenase gene promoter which substantially contains a base sequence represented by SEQ ID NO: 1, 48, 49 or 50; a formate dehydrogenase gene terminator which substantially contains the base sequence represented by SEQ ID NO: 2; a gene expression cassette which comprises the above-mentioned promoter, a foreign gene and the above-mentioned terminator; a recombinant expression vector which contains this gene expression cassette; a product of transformation with this recombinant expression vector; and a process for producing a foreign gene expression product which comprises incubating the transformant in a medium and taking up the foreign gene expression product from the culture medium thus obtained.
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机译:Boidinii的甲酸脱氢酶基因的启动子,其基本上含有从序列表中SEQ ID NO:1表示的碱基序列中挑选出来的190bp或以上的连续碱基序列;甲酸酯脱氢酶基因启动子,其基本上含有由SEQ ID NO:1、48、49或50表示的碱基序列;甲酸酯脱氢酶基因终止子,其实质上含有由SEQ ID NO:2表示的碱基序列;基因表达盒,其包含上述启动子,外源基因和上述终止子;含有该基因表达盒的重组表达载体;用该重组表达载体转化的产物;制备外源基因表达产物的方法,该方法包括将转化体在培养基中温育并从如此获得的培养基中吸收外源基因表达产物。
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