METHOD FOR EVALUATING ENZYME ACTIVITY IN OIL-POLLUTED SOIL AND MICROBIAL DECOMPOSITION METHOD USING THE SAME

摘要

PROBLEM TO BE SOLVED: To provide a method for the evaluation of enzyme activity in oil-polluted soil to easily perform the monitoring of soil microorganisms for confirming the effect of bioremediation in the microbial decomposition of the pollutant in the polluted soil and provide a microbial decomposition method using the evaluation method.;SOLUTION: The evaluation of enzyme activity is carried out by (101) collecting a specimen from oil-polluted soil, (103) weighing a prescribed amount of the minus-sieve fraction of the specimen and putting in a sterilized medium-size test tube, (104) adding a buffer solution and 0.3 ml of INT solution to the test tube and stirring with a mixer for 10 sec to obtain a treated substance, (105) stirring the treated substance in a sterilized medium-size test tube and carrying out static culture, (106) measuring the A₄₈₅ of the filtrate extracted from the cultured substance with a spectrophotometer, (107) separately preparing a standard curve from a standard INT formazan solution, (108) applying the measured result of A₄₈₅ of the filtrate to the standard curve to quantitatively determine the formazan in the filtrate, and (109) evaluating the dehydrogenase activity in the oil-polluted soil from the quantative data.;COPYRIGHT: (C)2005,JPO&NCIPI