首页> 外国专利> METHOD FOR QUANTITATIVELY DETERMINING TARGET NUCLEIC ACID, NUCLEIC ACID USED FOR THE SAME, PROBE-IMMOBILIZED SUBSTRATE, ASSAY KIT AND METHOD FOR DETECTING VIRUS BY USING THE SAME

METHOD FOR QUANTITATIVELY DETERMINING TARGET NUCLEIC ACID, NUCLEIC ACID USED FOR THE SAME, PROBE-IMMOBILIZED SUBSTRATE, ASSAY KIT AND METHOD FOR DETECTING VIRUS BY USING THE SAME

机译:定量测定目标核酸,用于相同目标的核酸,探针固定化的基质,测定试剂盒和使用相同目标检测病毒的方法

摘要

PROBLEM TO BE SOLVED: To provide a method for quantitatively determining a target nucleic acid, capable of simply determining the target nucleic acid in a sample, especially a virus genome or an mRNA, a nucleic acid used for the same, a probe-immobilized substrate, an assay kit and a method for detecting virus.;SOLUTION: This method for quantitatively determining the target nucleic acid is provided by competitively amplifying a sample nucleic acid and in a plurality of concentration standard substances containing sequences homologous to each of ≥2 target sequences contained in the target nucleic acid except for a part of the sequences, by using the target sequence, concentration standard substances corresponding to it and the same primer set, and determining the amplified substances obtained by the above for each of the kinds of the sequences.;COPYRIGHT: (C)2004,JPO
机译:解决的问题:提供一种定量测定靶核酸的方法,该方法能够简单地测定样品中的靶核酸,尤其是病毒基因组或mRNA,用于该靶核酸的核酸,固定有探针的底物解决方案:通过竞争性扩增样品核酸并在多种浓度标准物质中竞争性地提供这种定量确定靶核酸的方法,所述浓度标准物质中含有与≥ 2每个靶标同源的序列通过使用靶序列,与之对应的浓度标准物质和相同的引物组,对靶核酸中除序列的一部分以外的序列进行测序,并针对每种序列确定通过上述方法获得的扩增物。;版权:(C)2004,日本特许厅

著录项

  • 公开/公告号JP2004113194A

    专利类型

  • 公开/公告日2004-04-15

    原文格式PDF

  • 申请/专利权人 TOSHIBA CORP;

    申请/专利号JP20020284452

  • 申请日2002-09-27

  • 分类号C12N15/09;C12M1/00;C12Q1/68;G01N33/53;G01N33/566;G01N33/569;

  • 国家 JP

  • 入库时间 2022-08-21 23:33:27

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