Artificial chromosome or mini-chromosome that is based on the neo-centromere

摘要

Mammal (eg, a human) the present invention and non inter alia, and is related to nucleic acid molecules that are defined, including the hybrid its latent form, or composite type or a functional derivative thereof, or a neo-centromere in general or isolated their use in case of developing artificial chromosomes and mini-chromosomes of eukaryotes, including a range of artificial chromosomes and mini-chromosomes of mammals. The present invention provides telomere associated chromosome truncation to develop a mini-chromosomes (TACT) approaches, but is not limited to these approaches. The present invention also relates to transfection any approach that uses DNA previously created or cloned provide neocentromeres features in the construction of artificial chromosomes and relates truncation any approach to create a mini-chromosome containing the neo centromere. Artificial chromosomes and mini-chromosomes such are useful for gene therapy of certain range.