Quantitative assay for expression of genes in microarray

摘要

(Eg, a combinatorial library method for the detection in a microarray hybridization or gene expression have been developed, spots are arranged very densely amount is very low and, this is an uncomfortable situation The cause, where dense reaction may spill into adjacent spots). Therefore, uncertainty occurs in the system of the reaction of adjacent sites. This assay uses a digoxigenin enzyme assays for the detection. Methods for enhancing the reliability of the analysis of the expression of DNA in microarray format was developed. It uses a software analysis to normalize the spot. This process, to quantify automatically the large-scale array data using a modified template technology space despite possible destruction of the array. Each node in the deformable template represents the gene spot, I will repeat according to gradient descent rule. Minimizes the energy function, which merges the template deformation energy and data mismatch energy.