Cloning of oxidation reduction enzyme gene and the same gene and production manner null of the same

摘要

PROBLEM TO BE SOLVED: To obtain a new oligonucleotide, to provide a method for amplification and cloning of a new oxydoreductase gene using the oligonucleotide as a primer, to obtain a recombinant vector containing the gene and a transformant transformed by the recombinant vector, and to provide a method for producing an oxydoreductase using the transformant.;SOLUTION: The oligonucleotide is synthesized based on an oxydoreductase gene of Bacillus cereus FERM P-14210 that is provided by the inventors in the Patent Application Number 2000-257829, the new oxydoreductase gene is amplified by the PCR method using the oligonucleotide as a primer, and the recombinant vector containing the amplified product obtained by amplifying the new oxydoreductase gene by the PCR method is produced. Next, the transformant transformed by using the recombinant vector is cultured and the oxydoreductase is obtained by collection of the oxydoreductase from the culture.;COPYRIGHT: (C)2002,JPO