Method of detecting a substance that inhibits the function of the secretory protein and type III secretion mechanism of bacteria

摘要

Without depending on the experimental infection of the animals, the present invention can be easily detected in minutes a large amount of substances that specifically inhibit the function of type III secreted proteins secreted therefrom, as well as type III secretion mechanism is the detection method. That is, characterized by detecting a change in the hemolytic activity produced by mixing the erythrocyte suspension and the bacteria with a type III secretion mechanism, the addition of type III secretion mechanism inhibitor thereto, the type III secretion machinery of bacteria It is a method of detecting a substance that inhibits the. From a numerical value as an indicator hemolytic activity of red blood cells, the processing of many samples are possible in a short period of time, a substance that inhibits the function of type III secretory protein or type III secretion mechanism of bacteria, this detection method, pharmaceuticals It is advantageous to the development of.