A METHOD FOR DETECTING SUBSTANCES INHIBITING THE BACTERIAL TYPE III SECRETION MECHANISM AND FUNCTION OF SECRETORY PROTEINS THEREOF

摘要

The present invention is a detection method which can be easily detected in a large amount of a material that does not depend on the infection experiment animal, inhibiting the function of the type III secretion apparatus and the type III secreted proteins secreted from them specifically, and the short period of time. That is, the type III of the material to mix the bacteria and red blood cell suspension having a secretion mechanism, and this inhibits the type III secretion mechanism of the bacteria, characterized in that detecting a change in the type III secretion mechanism hemolytic activity occurs as imposing inhibitor in a detection method. This detection method is advantageously a substance that inhibits the function of a type III secretion mechanism, or type III secreted proteins in bacteria such as available from the processing of many samples in a short time by quantifying the red blood cells of the hemolytic activity as an index of drug development.