PREPARATION OF WEAKLY ACTIVE OR INACTIVE ALKALINE PHOSPHATASE MUTANT, AND ITS EXPRESSION IN YEAST

摘要

PROBLEM TO BE SOLVED: To provide a method for preparing a weakly active or inactive alkaline phosphatase mutant and a method for expressing the mutant in an yeast.;SOLUTION: The invention relates to a method for the recombinant production or expression of eukaryotic alkaline phosphatase (AP) mutants in yeast cells wherein the specifically introduced mutations result in a reduction in specific activity of AP by at least a factor 1:100. The invention also relates to a method for inserting the corresponding nucleic acid sequences into a vector for expression in methylotrophic yeast strains and it relates to the corresponding vectors and host strains.;COPYRIGHT: (C)2004,JPO