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PRODUCTION OF WEAKLY ACTIVE OR INACTIVE MUTANTS OF ALKALINE PHOSPHATASE AND THEIR EXPRESSION IN YEAST
PRODUCTION OF WEAKLY ACTIVE OR INACTIVE MUTANTS OF ALKALINE PHOSPHATASE AND THEIR EXPRESSION IN YEAST
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机译:弱碱性或碱性磷酸酶的产生及其在酵母中的表达
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摘要
The invention concerns a method for the recombinant production or expression of eukaryotic alkaline phosphatase mutants in yeast cells wherein the specifically introduced mutations result in a reduction of the specific AP activity by at least a factor 1:100. The invention also concerns a method for inserting corresponding nucleic acid sequences into a vector for expression in methylotrophic yeast strains and it concerns corresponding vectors and host strains.
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