An enzymatic assay for chlorite, chlorate and perchlorate determination based on horseradish peroxidase, chlorate reductase, or nitrate reductase and a reacting dye was developed. The assay is quick and simple requiring less than 30 min; is cheap costing less than $0.05 per sample; uses standard laboratory equipment; is sensitive to a lower limit of 0.4 micromolar and linear up to 6.0 millimolar; is highly reproducible and interference free. The application of this assay to chlorite analysis in treated water will significantly reduce one of the major costs associated with the use of chlorine dioxide as a disinfectant and improve the drinking water quality.
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