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Methods for comparitive analysis of carbohydrate polymers and carbohydrate polymers identified using same

机译:碳水化合物聚合物和使用该碳水化合物聚合物鉴定的碳水化合物聚合物的比较分析方法

摘要

Disclosed is a method for characterizing a carbohydrate polymer by identifying at least two binding agents that bind to the carbohydrate polymer. Binding is preferably determined by contacting the carbohydrate polymer with substrate that contains a plurality of first saccharide-binding agents affixed at predetermined locations on the substrate. The carbohydrate polymer is allowed to contact the substrate under conditions that allow for formation of a first complex between the first saccharide-binding agent and the carbohydrate polymer. A second saccharide-binding agent, which preferably includes a label, is also contacted with the carbohydrate polymer under conditions that allow for formation of a second complex between the second binding agent and the first complex. Identification of the first and second binding agent allows for characterization of the polysaccharide.
机译:公开了一种通过鉴定至少两种与碳水化合物聚合物结合的结合剂来表征碳水化合物聚合物的方法。优选通过使碳水化合物聚合物与含有固定在基质上预定位置的多种第一糖类结合剂的基质接触来确定结合。使碳水化合物聚合物在允许在第一糖结合剂和碳水化合物聚合物之间形成第一复合物的条件下接触底物。在允许在第二结合剂和第一复合物之间形成第二复合物的条件下,还将第二糖结合剂(其优选包括标记)与碳水化合物聚合物接触。第一和第二结合剂的鉴定可以表征多糖。

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