The present invention relates to a method for testing liver function by for example measuring levels of labelled CO2? in exhaled breath after providing a patient with doses of a labelled substrate capable of being metabolized by the liver to CO2, a substrate incapable of being metabolised by the liver CO2. The present invention also provides a kit based on the abovementioned method for testing liver function in a subjects. The method and kit of the present invention can be further applied to the non-invasive monitoring of disease status of a subject on a regular basis and to the detection of changes in liver function of a subject due to adverse drug reactions or chronic medication.
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