Chimeric GFP-aequorin as bioluminescent Caamp;plus;amp;plus; reporters at the single cell level

摘要

A modified bioluminescent system comprising a fluorescent molecule covalently linked with a photoprotein, wherein said link between the two proteins has the function to stabilize the modified bioluminescent system and allowing the transfer of the energy by Chemiluminescence Resonance Energy Transfer (CRET).