Method of generating an expression profile of estrogen receptor isoforms and related methods

摘要

A method of amplifying and quantifying a mRNA transcript of the or isoform of the ER; a method of generating an expression profile of mRNA transcripts of the or isoform of the ER, wherein the mRNA transcripts comprise wild-type or alternatively spliced exons, comprising reverse-transcribing the mRNA transcript, amplifying the resulting cDNA using a targeted primer and a partner primer, contacting the resulting amplicons with a probe, detecting the probe, and quantifying the detected probe; isolated oligonucleotides to be used as a targeted primer, partner primer, or probe; a method of determining a hyperproliferative condition or a predisposition to a hyperproliferative condition, such as breast cancer or a risk for breast cancer; a method of screening candidate therapeutic treatments for an ER-sensitive condition; and a method of prognosticating response of a mammal to a therapeutic treatment of breast cancer; and other methods.