Nucleic acid amplification with DNA-dependent RNA polymerase activity of RNA replicases

摘要

The present invention entails methods and kits for carrying them out based on the discovery that an RNA replicase, such as Q replicase, has DNA-dependent RNA polymerase (DDRP) activity with nucleic acid segments, including DNA segments and DNA:RNA chimeric segments, which comprise a 2-deoxyribonucleotide or an analog thereof and which have sequences of RNAs that are autocatalytically replicatable by the replicase.